Channa striata is a native fresh water carnivorous air breathing fish species, widely distributed in India. This white boneless meaty and tender taste edible fish is selected for the present study. The aim of the present study was to determine the impact of organophosphate pesticide, Chlorpyrifos on the gills and liver of C. striata. Acute toxicity test results for pesticides were evaluated by the Probit analysis method and 96 h LC50 values for C. striata exposed to chlorpyrifos was 4.521 ppm. Histology of liver and gills exposed to 96-hour LC50 concentration of chlorpyrifos was studied in a fresh water Channa striata. The histopathological changes observed in the liver tissue included necrosis, degradation of hepatocytes, degeneration of blood vessels, distended sinusoids with pyknotic nuclei and vacuolation of cells.